Detection of acid-fast bacilli in concentrated primary specimen smears stained with rhodamine-auramine at room temperature and at 37 degrees C

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Detection of acid-fast bacilli in concentrated primary specimen smears stained with rhodamine-auramine at room temperature and at 37 degrees C.

Many laboratory workers prefer the rhodamine-auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rhodamine-auramine stain. A total of 782 primary smears were evaluated blindly by...

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Evaluation of sputum smears concentrated by cytocentrifugation for detection of acid-fast bacilli.

Early identification and isolation of tuberculosis patients is of utmost importance to minimize the risk of further epidemic spread of the disease. The traditional concentrated acid-fast smears are not very reliable tools for the presumptive diagnosis of tuberculosis. Acid-fast bacillus (AFB) smears from 120 patients specimens and 80 simulated AFB samples were processed according to standard la...

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Incidence and significance of acid-fast bacilli in sputum smears at the end of antituberculous treatment.

OBJECTIVE A group of patients with pulmonary tuberculosis (PT) who received and correctly completed antituberculous therapy were studied to determine the incidence and significance of positive acid-fast bacilli (AFB) in sputum smears at the end of this treatment. DESIGN Retrospective chart review of persons with bacteriologically proved PT. PATIENTS Of 1,052 patients diagnosed as having PT ...

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Relationship between CT Findings of Pulmonary Tuberculosis and Acid-Fast Bacilli Sputum Smears

Background and purpose: Computed tomography (CT) scan is of great help in confirming the diagnosis of tuberculosis (TB) before the sputum test result. The purpose of this study was to investigate the relationship between pulmonary involvement patterns in CT scans of TB patients with positive sputum smears. Materials and methods: This retrospective descriptive-analytical study was conducted usi...

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Vitamin C as a green and efficient catalyst in synthesis of quinoxaline derivatives at room temperature

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1994

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.32.10.2487-2489.1994